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. Author manuscript; available in PMC: 2012 Nov 27.
Published in final edited form as: J Biochem Mol Toxicol. 2010 Sep-Oct;24(5):330–334. doi: 10.1002/jbt.20342

Figure 3. Effect of HNE on metallothionein transcription.

Figure 3

COS-7 cells were transfected with metallothionein reporter genes: −153/+62-CAT, MREd5-CAT, and ARE4-CAT. Following transfection and recovery, cells were exposed to 10 μM HNE for 24 h (solid bars). The level of CAT was then measured by ELISA and normalized to the amount of β-galactosidase enzyme activity (11). Reporter gene expression in cells not exposed to HNE (open bars) in each treatment, n = 3 observations. * indicate significant difference at p < 0.01.