Table 1.
Primary and Secondary Antibodies
| Antibody name | Specificity | Host species | Dilution rates | Company |
|---|---|---|---|---|
| Nestin | Neural stem cells | rabbit | 1:200 | Millipore |
| Musashi-1 | Neural precursor cells | rabbit | 1:200 | Millipore |
| Tuj-1 | Newborn neurons | mouse | 1:1000 | Millipore |
| GFAP | Astrocytes | rabbit | 1:1000 | Millipore |
| Active caspase-3 | Apoptotic cells | rabbit | 1:10 | Millipore |
| BrdU | Newly-generated cells | mouse | 1:1000 | Millipore |
| Ki67 | Proliferative cells | rabbit | 1:1000 | Millipore |
| NR1 | NMDA receptor subunit 1 | rabbit | 1:200 | Cell Signaling |
| NR2A | NMDA receptor subunit 2A | rabbit | 1:200 | Millipore |
| NR2B | NMDA receptor subunit 2B | rabbit | 1:200 | Millipore |
| rabbit IgG Alexa-594 | Specific to rabbit primary antibodies | goat | 1:1000 | Invitrogen |
| mouse IgG Alexa-488 | Specific to mouse primary antibodies | goat | 1:1000 | Invitrogen |