Figure 2. Upon coculture with MCF7 cells, fibroblasts overexpressing BDH1 or HMGCS2 show Cav-1 downregulation. Fibroblasts overexpressing BDH1, HMGCS2 or empty vector control were co-cultured with MCF7 cells for 5 d. Then, cells were fixed and immunostained with anti-Cav-1 (red) and anti-K8–18 (green) antibodies. Nuclei were stained with DAPI (blue). Cav-1 staining is shown in the top panels to better appreciate that upon coculture with MCF7 cells, fibroblasts overexpressing BDH1 or HMGCS2 show downregulation of Cav-1, as compared with MCF7 cells cocultured with control fibroblasts. Original magnification, 40x.