Figure 4. Coculture with fibroblasts overexpressing BDH1 or HMGCS2 increases the MCF7 cell population. GFP (+) MCF7 cells were grown in co-culture with fibroblasts harboring BDH1, HMGCS2 or the empty vector control for 5 d. Then, cells were trypsinized and analyzed by flow cytometry using a 488 nm laser, to identify the GFP (+) MCF7 cell population. Fibroblasts were identified as the GFP (-) cell population. (A) Traces of the relative percentage of MCF7-GFP (+) cells and GFP (-) fibroblasts. (B) Histogram representation of the relative percentage of MCF7 cells and fibroblasts. The presence of fibroblasts overexpressing BDH1 and HMGCS2 increases the proportion of MCF7 cells by > 2-fold. *p < 0.0002.