Figure 6. Generation of MDA-MB-231 cells overexpressing enzymes for ketone utilization. MDA-MB-231 breast cancer cells overexpressing ACAT1, ACAT2, OXCT1, OXCT2 or LV-105 empty vector control were generated using a lentiviral approach. Western blot analysis was performed with isoform-specific antibodies to confirm the overexpression of ACAT1 (A), ACAT2 (B), OXCT1 (C), OXCT2 (D), relative to empty vector control cells. Equal loading was assessed with β-actin.