Figure 4. Treatment with CsA and Rapa does not induce cell death but leads to a small delay in cell cycle progression. Analysis of total DNA content of CACO-2 cells cultured in control conditions or treated with CsA (2 μM), FK506 (2 μM) or Rapa (20 nM). At indicated time-points cells were collected and their nuclei DNA content analyzed by propidium iodine (PI) staining and FACS in logarithmic scale to favor SubG0 (A) or in linear scale to favor 2–4 n DNA content analysis (B). Data are representative of six independent experiments.