Figure 6. Treatment with CsA induces necroptosis. (A) CACO-2 cells were treated with vehicle, CsA, FK506, Rapa or a combination of CsA and Nec-1 as depicted in the figure for 48h. Data are the average of four independent experiments. (B) CACO-2 cells were cultured in the presence of drugs as described in A for 4 d. At each time point samples were trypsinized and counted (upper panel) or fixed and stained with violet crystal (lower panel). Results are the mean of three independent experiments. * marks p < 0.05 when comparing to group treated only with CsA, in the absence of Nec-1.