Figure 9. CsA blockade of CACO-2 cell growth is not mediated by altered NFκB activity. CACO-2 cells were transfected with pGL3–6xNFκB-Luc and pRL-TK. Twenty-four hours after transfection, cells were treated overnight with CsA (2 μM), FK506 (2 μM), Rapa (20 nM), Ly294002 (30 μM) or MG132 (20 μM). Cells were stimulated for the last 6 h of culture with PMA (20 nM), lysed and luciferase activity was measured. Results are the mean of two independent experiments. * marks p < 0.05 when comparing to unstimulated group.