Figure 2. Effect of B-Myb knockdown on S-phase progression. (A). HepG2 cells transfected with B-Myb-specific and control siRNAs were synchronized by mimosine treatment. The cells were then released into S-phase for the indicated times, followed by flow cytometry to examine the progression through S-phase. The white and black arrows mark the G₁ and G₂ DNA content, respectively. (B). The successful knockdown of B-Myb was confirmed by western blotting.