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. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: Mol Cancer Ther. 2012 Sep 18;11(11):2311–2320. doi: 10.1158/1535-7163.MCT-12-0009

Figure 5. Ectopic expression of recombinant PTK6 rescues DNA damage-induced apoptosis in PTK6 stable knockdown cells.

Figure 5

(A) Endogenous PTK6 detected by immunoblotting of lysates prepared from pLKO.1 vector and shRNA49 (PTK6 shRNA) stable HCT116 cell lines. (B) Recombinant PTK6 or pcDNA3 vector was introduced by transient transfection into HCT116 cells stably expressing PTK6 shRNA or PLKO1 vector (control). Cells were treated for 24 hours with 10 uM doxorubicin, 300 mM 5-FU, or DMSO control. Immunoblotting was performed using antibodies against PTK6, cleaved Caspase 3 and cleaved PARP; β-actin was examined as a loading control. (C) The ratios of cleaved Caspase 3 and cleaved PARP relative to β-actin in control vector (pcDNA3) and PTK6 knockdown cells (PTK6 shRNA) were quantified using NIH ImageJ (* P < 0.05, bars +/− SD).