FIG. 1.

Gene editing model system and expression of enhanced green fluorescent protein (eGFP). (A) The mutant sequence of eGFP integrated in HCT116-19 with the nucleotide targeted for change is listed. The DNA oligonucleotide (ODN) is 72NT, a 72-mer with 3 phosphorothioate linkages on each of the termini. Bold indicates the targeted codon, with asterisks indicating the position of phosphorothioate linkages. (B) HCT116-19 cells (1×10⁶) containing a single integrated copy of the wild-type eGFP gene were plated and the expression of eGFP was monitored over the course of 168 hours. The percentage of cells expressing eGFP was calculated using fluorescent activated cell sorting (FACS) analysis.