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. 2012 Dec;22(6):405–413. doi: 10.1089/nat.2012.0374

FIG. 3.

FIG. 3.

Optimization of ODN delivery into HCT116 cells using nucleofection. HCT116-19 cells were transfected with an Alexa Fluor 488–tagged ODN in appropriate nucleofector solution (solution SE, SF, or SG). (A) For each nucleofector solution, 15 programs were tested (x-axis). Cells were allowed to recover in a 24-well plate with complete growth media for 24 hours, and the transfection efficiency was measured by FACS. Transfection efficiency was measured as the percentage of viable Alexa Flour 488 positive cells to the total viable population as measured by FACS analysis. (B) The top 7 programs were chosen and HCT116-19 cells were transfected with the Alexa Flour 488 ODN to determine the best solution/program combination to use with the HCT116 cell line.