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Gene editing activity in cells with nucleofection as a delivery mode. HCT116-19 cells were synchronized with 6 μM aphidicolin for 24 hours, released for 4 hours, and then targeted by nucleofection at 5 μM, 10 μM, 15 μM, and 20 μM concentrations of 72NT. Cells were analyzed using FACS 24 hours after targeting. Correction efficiency was calculated as the number of live eGFP positive cells divided by number of total live cells in the population.
