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Levels of gene editing over an extended timeframe. HCT116-19 cells were synchronized with 6μ M aphidicolin for 24 hours, released for 4 hours, and targeted using nucleofection with 20 μM 72NT. Cells were allowed to recover for 1, 2, 4, 5, 6, 7, or 8 days prior to FACS analysis. Correction efficiency was determined as number of eGFP positive cells divided by total number of live cells in the population.
