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. 2012 Dec;22(6):428–437. doi: 10.1089/nat.2012.0380

FIG. 2.

FIG. 2.

(A) 8% Native-polyacrylamide gel electrophoresis (PAGE) gel of unmodified pRNA-3WJ-(MGapt) and pRNA-3WJ-(MGapt) made with 2′F ATP and CTP in the presence of RPMI 1640 medium containing 10% fetal bovine serum (FBS) stained with both ethidium bromide and MG dye to show that both forms of the aptamer are capable of binding MG but are rapidly degraded in the presence of FBS. (B-C) 8% Native-PAGE gels showing pRNA (B) or pRNA-3WJ-(MGapt) (C) made with either 2′F UTP and CTP or 2′F ATP and CTP in the presence of RPMI 1640 medium containing 10% FBS for up to 4 hours. In both cases, the unmodified RNA is degraded within the first hour of exposure to FBS, whereas the 2′F-modified strands remain stable. For all gels, lane 6 contains a DNA ladder. RPMI, Roswell Park Memorial Institute.