Table 1.
Dosage and immunization schedule of female BALB/c mice immunized with monovalent formulation of either HPV16 or HPV18 VLPs expressed in Pichia pastoris. Last column shows the geometric mean titer of serum antibody in immunized mice. Range of antibody titers in each group is mentioned in parenthesis.
| Group | Treatment (VLP) | Dose
|
No of mice | Immunization days | Bleeding days | GMT Serum antibody (range) | |
|---|---|---|---|---|---|---|---|
| Quantity (in μg) | Number of doses | ||||||
| 1 | HPV16 | 2 | 3 | 7 | 0, 14 and 28 | 0, 14, 28 and 35 | 64,000 (16,000–128,000) |
| 2 | HPV16 | 10 | 3 | 7 | 0, 14 and 28 | 0, 14, 28 and 35 | 47,551 (8000–256,000) |
| 3 | HPV16 | 20 | 3 | 7 | 0, 14 and 28 | 0, 14, 28 and 35 | 105,002 (64,000–256,000) |
| 4 | HPV18 | 1 | 3 | 7 | 0, 14 and 28 | 0, 14, 28 and 35 | 78,016 (16,000–256,000) |
| 5 | HPV18 | 3 | 2 | 7 | 0 and 28 | 0, 14, 28 and 35 | 172,275 (128,000–256,000) |
| 6 | HPV18 | 5 | 3 | 7 | 0, 14 and 28 | 0, 14, 28 and 35 | 95,103 (32,000–256,000) |
| 7 | Placebo | Al-Hydroxide | 3 | 7 | 0, 14 and 28 | 0, 14, 28 and 35 | 0 |