Fig. 3.

Binding of the N_PTX3 mutants to IαI and TSG-6. Dose-response binding of different amounts of IαI (A) or TSG-6 (B) to immobilized wild type N_PTX3 (WT) and its single (C103), double (C47/C49), and triple (C47/C49/C103) mutants. All the assays were performed at pH 7.4 in the presence of PBS with Ca²⁺ and Mg²⁺. The extent of binding was determined by enzyme-linked immunosorbent assay. Values of four independent experiments done in duplicate are plotted (mean ± S.D.).