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Effect of exogenous FGF2 on cumulus expansion. Micrographs showing the morphology of (A) wild type COCs cultured for 16 h with 100 ng/ml FSH, 2% FBS in the absence and in the presence of 100 ng/ml FGF2; (B) Ptx3 null COCs cultured with 100 ng/ml FSH, 2% FBS without or with either 3 nM full length recombinant PTX3 (Mw = ~ 340 kDa) or 14 nM recombinant N_PTX3 (Mw = ~ 70 kDa) in the presence of FGF2 (Mw = ~ 17.5 kDa).
