Figure 3.
CD103+ intestinal DCs express elevated levels of the TGFβ-activating integrin αvβ8 and activate more TGF-β in an integrin αvβ8-dependent manner. RNA from (A) total DCs or (B) splenic/mLN CD103+/− DCs was analyzed for integrin β8 expression by qPCR. β8 levels were normalized to the housekeeping gene HPRT and presented relative to levels in total spleen DCs (for A) or spleen CD103-ve DCs (for B). Data shown are representative of 2 and 4 independent experiments, respectively. (C) TGF-β activation by control or αvβ8−/− CD103+/− mLN DCs was detected by coculture with an active TGF-β reporter cell line.12 Data represent 5 independent experiments. (D) RNA from control or αvβ8−/− CD103+/− DCs was analyzed for TGF-β expression by qPCR. Data shown represent 2 independent experiments. *P < .05, **P < .01.