Table 1.
Summary of the materials used in the EST analysis
| Library | Prefix | Plate number | cDNA amplification | Sequence primer | DNA sequencer | Valid 5' reads | Valid 3' reads | Overlap contigs | Full insert sequences |
|---|---|---|---|---|---|---|---|---|---|
| Eye |
Dj_aE |
000 |
Plasmid |
SK |
ABI 3700 |
918* |
- |
- |
- |
| Head |
Dj_aH |
000 |
Plasmid |
SK, M13 |
ABI 3700 |
6,444* |
3,163 |
689 |
495 |
| Head |
Dj_aH |
001 - 022 |
Plasmid |
SK |
ABI 3700 |
5,024 |
- |
- |
21 |
| Head |
Dj_aH |
101 - 140 |
Plasmid |
SK |
ABI 3700 |
2,364 |
- |
- |
4 |
| Head |
Dj_aH |
201 - 227 |
TempliPhi |
SK |
ABI 3730xl |
8,426 |
- |
- |
52 |
| Head |
Dj_aH |
301 - 327 |
TempliPhi |
SK, M13 |
ABI 3730xl |
8,366 |
7,107 |
4,516 |
21 |
| Head |
Dj_aH |
401 - 406 |
TempliPhi |
T3 |
ABI 3730xl |
2,056 |
- |
- |
- |
| Head |
Dj_aH |
501 - 530 |
TempliPhi |
T3, M13 |
ABI 3730xl |
9,462 |
8,191 |
5,888 |
- |
| Total | 43,060 | 18,461 | 11,093 | 593 |
* DDBJ entries registered by previous research.