Figure 5.

EMSA to assess the interaction of C. acetobutylicum CcpA with (A) xylB promoter region PxylB, (B) araE1 promoter region ParaE1 and (C) upstream region (non-coding region, Psol) of the sol operon. His₆-CcpA with different concentrations (0–0.8 μM, 0–0.6 μM and 0–0.6 μM for PxylB, ParaE1 and Psol, respectively) and 2 nM of Cy5-labelled PCR fragments were used. The PCR fragment of gene CAC1790 was used as the non-specific competitor. Abbreviations: Spec. comp., specific competitor; Nonspec. comp., non-specific competitor.