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. 2012 Nov 27;7(11):e50180. doi: 10.1371/journal.pone.0050180

Figure 3. MALDI-TOF imaging of whole eye section of a rabbit instilled twice a day with one drop of 0.01% benzalkonium chloride (BAK) for 5 months.

Figure 3

MALDI-TOF imaging shows the BAK distribution in a BAK-treated eye. (a) Histology image of an adjacent cryosection stained with hematoxylin-eosin (HE) showing three areas of interest: cornea (area 1), nasal iridocorneal angle (area 2) and optic nerve area (area 3). (b, c) Overlays between HE staining and MALDI-TOF ion images of BAK C12 and C14 distributions in whole eye section at m/z 304.30 and 332.33, respectively. (d, e) MALDI-TOF ion images of BAK C12 and C14 distributions at m/z 304.30 and 332.33, respectively. Intensities of the ions are represented in colour, based on the intensity scale provided (from black to white). Field of view 16×15 mm. (f, g, h) MALDI-TOF mass spectra extracted from areas 1, 2 and 3, respectively, showing BAK C12 and C14 ion peaks.