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. 2012 Nov 27;7(11):e50480. doi: 10.1371/journal.pone.0050480

Table 1. Purification scheme of the La3+-dependent MDH isolated from M. extorquens strain AM1.

Step Total activity (Unit) Specific activity (U/mg) Purification (fold) Yield (%)
Cell free extract 46 0.62 1.0 100
PD-10 33 0.74 1.2 71
Hi-trap SP HP Sepharose HP 15 14 22 32
MonoS 5/50 GL 4.5 10 17 18