Table 3.

Relative gene expression in the muscle tissue of chickens treated with different levels of AgNano

Gene symbol	Treatment					ANOVA
	Control	10 ppm AgNano	20 ppm AgNano	10 ppm AgNanoc	20 ppm AgNanoc	SEM	P value
FGF2a	0.67†	1.28‡	1.44‡	1.05‡	1.23‡	0.070	0.0003
FGF2b	0.60†	0.98	1.38‡	0.95	0.93	0.068	0.0024
VEGFAa	1.39	1.08†	1.03†	1.13	1.60‡	0.064	0.0057
VEGFAb	1.28	1.02†	0.95†	1.01	1.28‡	0.055	0.0072

^aNormalised to ACTB; ^bnormalised to EEF1A2; ^ceggs were in ovo injected; ^†,‡different symbols indicate statistically significant differences (P < 0.05). AgNano, nanoparticles of colloidal silver; ANOVA, analysis of variance; SEM, standard error of the mean.