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. 2012 Nov 15;5:34. doi: 10.1186/1757-2215-5-34

Figure 4.

Figure 4

The anti-apoptotic effect of ascites cannot be reversed by OPG neutralizing antibodies. (A) CaOV3 cells were challenged with increasing concentrations of TRAIL and OPG (25 ng/ml) in the presence or absence of anti-OPG antibodies (10 μg/ml) for 48 h. Cell viability was determined by XTT assay and expressed as the percentage relative to control (untreated) cells. CaOV3 cells were challenged with TRAIL, OVC508 ascites (10%) (B) or OVC551 ascites (10%) (C), in the presence or absence of anti-OPG antibodies (10 μg/ml) for 48 h. Cell viability was assessed as above. (D) OVC508 ascites was incubated with anti-OPG antibodies (10 μg/ml) for up to 6 h. Protein G agarose was then added for 1 h and centrifuged and OPG levels were determined in the supernatant by ELISA. (E) CaOV3 cells were challenged with TRAIL (10 ng/ml), ascites (10%) and anti-OPG (10 μg/ml) for 48 h. The cells were washed and fresh medium was added. Viable colonies were counted after 14 days and data were expressed as% of colonies in control (untreated) cells. Data are expressed as means of triplicates from three independent experiments ± SD. NS indicates that differences did not reach statistical significance with P > 0.05.