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. 2012 Nov 27;7(11):e50301. doi: 10.1371/journal.pone.0050301

Figure 1. Estrogen administration causes an FSH-independent increase in vertebral bone mass.

Figure 1

A) Gross anatomy of uteri and serum levels of estrogen in Fshb+/+ and Fshb−/− mice 4 weeks after sham operation (−E2) or subcutaneous implantation of an estrogen pellet (+E2). B) Representative contact radiographs of the lumbar spine of the respective groups. C) Von Kossa/van Gieson-staining of non-decalcified spine sections and D) quantification of the trabecular bone volume per tissue volume (BV/TV), trabecular numbers (Tb.N), trabecular thickness (Tb.Th), and trabecular spacing (Tb.Sp). All data represent mean ± SD from at least 5 mice per group. *p<0.05 versus untreated control of each genotype.