. 2012 Nov 27;7(11):e50489. doi: 10.1371/journal.pone.0050489

Table 1. Thermodynamic parameters calculated from pressure unfolding curves of OBP at 25°C in the presence of 1.5 M GdnHCl.

Recorded by increasing pressure
Dimer	ΔV, ml.mol⁻¹	ΔGu, kJ.mol⁻¹	P_1/2, MPa
Csm	−34.18+/−1.24	8.37+/−0.34	245
fluorescence intensity	−58.54+/−3.59	11.98+/−0.97	204
ANS fluorescence	−45.92+/−4.52	7.11+/−1.36	154
*Dimer+ligand*
Csm	−172.60+/−5.80	57.56+/−1.93	333
Fluorescence intensity	−138.70+/−8.95	44.69+/−2.90	322
ANS fluorescence	−114.10+/−9.10	35.19+/−4.00	308
*Monomer*
Csm	−124.6+/−5.53	13.20+/−0.83	105
Fluorescence intensity	−88.98+/−8.27	11.96+/−1.20	134
ANS fluorescence	−92.17+/−4.44	10.73+/−0.57	116
*Monomer+ligand*
Csm	−82.68+/−6.22	29.15+/−2.12	352
Fluorescence intensity	−88.73+/−2.09	28.65+/−2.09	323
Recorded by decreasing pressure
*Dimer*	ΔV, ml.mol⁻¹	ΔGu, kJ.mol⁻¹	P_1/2, MPa
Csm	−61.47+/−2.95	4.52+/−0.02	73.53
fluorescence intensity	−78.20+/−3.75	4.76+/−0.2	60.86
*Dimer+ligand*
Csm	− 95.71+/−9.99	12.63+/−1.21	131.96
fluorescence intensity	−72.58+/−9.97	8.52+/−1.27	117.38
*Monomer*
Csm	−141.90+/−15.61	11.96+/−1.36	84.28
*Monomer+ligand*
Csm	−103.30+/−10.68	12.04+/−1.32	116
fluorescence intensity	−96.97+/−15.41	9.89+/−1.93	102