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(A) Amino acid sequences of [V7]20and [RGD-V6]20. (B) SDS-PAGE analysis of [RGD-V6]20 purification by inverse transition cycling at 42°C and 4°C. (C) UV-visible spectrum of [RGD-V6]20 in PBS. PS shows MWs of protein standards from top to the bottom: 200, 116, 97, 66, 45, 31, 22 and 14 kDa. CL, S and P represent cell lysate, supernatant and pellet, respectively.
