Figure 2.
Dependence of N2a cell adhesion on protein coating concentration. Protein-coated surfaces were prepared using 0.1, 0.2, 0.5, 1, 2, 5 or 10 μM of [V7]20, [RGD-V6]20 and fibronectin. Protein concentration was prepared based on the calculated MWs of 58.2, 59.6 and 225 kDa for [V7]20, [RGD-V6]20 and fibronectin. A405 values of samples were subtracted by 0.035 ± 0.004 of background control and used to generate cell adhesion graphs. * and # correspond to P < 0.05 and P < 0.01 from [V7]20, respectively.