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. 2012 Nov 27;7(11):e50668. doi: 10.1371/journal.pone.0050668

Figure 2. TLRs signaling are required for HMGB1-mediated TNF-α and IL-1β expressions in KCs of burn rats.

Figure 2

After 48-h-stimulation with 100 ng/ml HMGB1, KCs released significantly higher levels of TNF-α and IL-1β than those of unstimulated. Pre-incubation of KCs with anti-TLR2 or anti-TLR4 antibody markedly suppressed the release of TNF-α and IL-1β in the HMGB1-stimulated KCs harvested from burn rats. (A) TNF-α release by KCs from burn rats. (B) IL-1β release by KCs from burn rats. Results were given as mean±SEM (n = 8). After burn injury, the KCs treated with medium or HMGB1 alone were used as unstimulated or positive control respectively. * = p<0.05, ** = p<0.01 vs. unstimulated controls, ANOVA; ## = p<0.01, vs. positive controls, ANOVA.