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. 2012 Nov 27;7(11):e50668. doi: 10.1371/journal.pone.0050668

Figure 3. TLR2 and TLR4 involve in HMGB1-induced expressions of TNF-α and IL-1β mRNAs in KCs from burn rats.

Figure 3

Expressions of TNF-α and IL-1β mRNAs were measured by Northern blot analysis. Relative mRNA levels were quantified by densitometry and expressed as OD ratio with GAPDH served as internal standards. HMGB1 showed a stimulatory effect on TNF-α and IL-1β mRNA levels in KCs. Pre-incubation of KCs with anti-TLR2 or anti-TLR4 antibody significantly attenuated HMGB1-induced TNF-α and IL-1β mRNA expressions in KCs from burn rats. (A) A representative result. (B) Results of TNF-α mRNA expression from the independent experiments. (C) Results of IL-1β mRNA expression from the independent experiments. Results were given as mean±SEM (n = 3). After burn injury, the KCs treated with medium or HMGB1 alone were used as unstimulated or positive control respectively. ** = p<0.01 vs. unstimulated controls, ANOVA; ## = p<0.01, vs. positive controls, ANOVA.