Table 2. Mutation spectrum within tetranucleotide repeats.
| cells | clone | M0 | M1 | M2 | ||||||||||
| −1 | 0 | −2 | −1/−2 | −1 | 0/−1 | 0 | 0/1 | 1 | −2 | −1 | 0 | 1 | ||
| HCT116 | [AAAG]17.1 | 14 | 12 | 8 | 5 | 2 | 1 | 21 | 3 | |||||
| [AAAG]17.2 | 10 | 9 | 3 | 6 | 5* | |||||||||
| HCT116+chr3 | [AAAG]17.1 | 3 | 13 | 1 | 1 | 4 | 1 | 5 | 7 | 1 | 1 | 6 | ||
| [AAAG]17.2 | 7 | 3 | 2 | 2 | 10 | 6 | 1 | 11 | ||||||
−2, −1, 1. Change in the number of [AAAG]-units (e.g. −2 = loss of 8 bp or [AAAG]2).
−1, 0: Wildtype or frameshift mutant cells without expected EGFP fluorescence.
−2, 1: In-frame mutant cells with expected EGFP fluorescence.
−1/−2, 0/1: Heteroduplex mutant cells with expected partial (dim) EGFP fluorescence.
*
including one mutant with a 2 bp (AA) deletion.