Table 1. Summary of RNA-seq results.
1 dpi | 4 dpi | 14 dpi | |||||||
carcasses | midguts | carcasses | midguts | carcasses | midguts | salivary glands | |||
Mappinga | DENVI | No.Mosquitoes | 30 | 25 | 40 | 40 | 40 | 40 | 40 |
No. reads | 45,440,807 | 15,516,385 | 42,994,979 | 19,370,451 | 45,741,611 | 21,238,385 | 19,120,751 | ||
B | No.mosquitoes | 30 | 30 | 30 | 30 | 20 | 20 | 20 | |
No. reads | 44,100,971 | 14,875,045 | 45,026,868 | 18,529,469 | 42,659,325 | 21,336,666 | 20,258,036 | ||
Differential transcript accumulationb | DEseq | 13 | 31 | 63 | 96 | 73 | 14 | 257 | |
Cufflinks | 369 (886) | 774 (1063) | 209 (537) | 563 (934) | 325 (567) | 498 (841) | 519 (861) | ||
Common | 10 | 30 | 57 | 94 | 69 | 13 | 190 |
Provides the number of mosquitoes dissected for each sample after either an infectious (DENVI) or non-infectious (B) bloodmeal in specific tissues at discrete days post infection (dpi). All mapping was to the Ae. aegypti transcriptome (AaegL1.2 gene build).