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. 2012 Nov 27;7(11):e50512. doi: 10.1371/journal.pone.0050512

Table 1. Summary of RNA-seq results.

1 dpi 4 dpi 14 dpi
carcasses midguts carcasses midguts carcasses midguts salivary glands
Mappinga DENVI No.Mosquitoes 30 25 40 40 40 40 40
No. reads 45,440,807 15,516,385 42,994,979 19,370,451 45,741,611 21,238,385 19,120,751
B No.mosquitoes 30 30 30 30 20 20 20
No. reads 44,100,971 14,875,045 45,026,868 18,529,469 42,659,325 21,336,666 20,258,036
Differential transcript accumulationb DEseq 13 31 63 96 73 14 257
Cufflinks 369 (886) 774 (1063) 209 (537) 563 (934) 325 (567) 498 (841) 519 (861)
Common 10 30 57 94 69 13 190
a

Provides the number of mosquitoes dissected for each sample after either an infectious (DENVI) or non-infectious (B) bloodmeal in specific tissues at discrete days post infection (dpi). All mapping was to the Ae. aegypti transcriptome (AaegL1.2 gene build).

b

Lists the number of genes identified by DEseq [35], Cufflinks [36] and common to both with transcripts accumulating differentially in the samples. The numbers in parentheses are the total number of transcripts isoforms detected as significantly differentially accumulated by Cufflinks.