Figure 1.
ATAF2 binds DNA sequences from the genomic pull-down assay. (A) Schematic representation showing ATAF2 and ∆ATAF2 deletion constructs. NAC domains C and D cover the DNA binding domain. (B) EMSA assay confirming ATAF2 and not ∆ATAF2 binds to DNA clones identified in genomic pull-down assays. DNA probes were prepared by PCR-amplification followed by end-labeling with [γ-32P]ATP. The asterisk indicates the shifted band after ATAF2 binding. The four clones tested (C7, C34, C52, and C113) were all located within 1000 bp upstream of the coding sequences of At1G08540, At1G68907, At3G26540, and At3G11700 respectively.