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. 2012 Aug 31;12:157. doi: 10.1186/1471-2229-12-157

Figure 2.

Figure 2

ATAF2 induces the transcription and binds to a 150-bp fragment within the promoter region of a defensin-like family protein (At1g68907). (A) Real-time qRT-PCR showing enhanced expression of At1g68907 in two 35S::ATAF2-GFP overexpression lines and in wounded Arabidopsis Shahdara plant tissue. RNA was extracted from five independent test plants. The data represents the average ± standard deviation from duplicate qRT-PCR reactions. (B) Schematic representation of the At1g68907 promoter fragment investigated for ATAF2 binding activity. EMSA assay showing ATAF2 binds to the DNA fragments F300-2 (C) and 150–3 (D) in the promoter region of At1G68907. The asterisk indicates the shifted band after ATAF2 binding.