Table 1.
Primers used in this study
| Name | Sequence (5’-3’) | Comments |
|---|---|---|
| NbTC9992-1F |
GCTGTCAAAGAAGTGTCATTA |
Specific primer for TC9992 |
| NbTC9992-1280R |
ACCGTTTATTAATCACTATATTGC |
Specific primer for TC9992 |
| NtBP1333-1 F |
CTTAATGGGCAAGCAGCTAATC |
Specific primer for BP133312 |
| NtBP1333-447R |
TCAAGATTGTATGTTGTCTGCTC |
Specific primer for BP133312 |
| LeBI9315-123F |
GTTGCAATTAAACAAGTTTCTCTGGA |
Specific primer for BI931567 |
| LeBI9315-658R |
GGCTGAAGATCATAGTACGG |
Specific primer for BI931567 |
| NbTC9992-458R-5RACE |
GCTTGTCCATAGCCTTGGTTCTTCCT |
5’-RACE for TC9992 |
| NbTC9992-49R-5RACE |
TTTGCCTTCCCCCATCGCCTTGAT |
5’-RACE for TC9992 |
| NbTC9992-F1 |
GGATTGGAAAGGGGGAACCT |
Sequencing |
| NtMAP3Kb-566F |
TCCGCCGGTCATGTCACT |
Sequencing |
| NtBP1333-199R-5RACE |
ACATGGCTGCAGCTGCTTCATATTC |
5’-RACE for BP133312 |
| NtBP1333-91R-nested |
TACTATCCTTCTGCATAACTGACTGCAA |
5’-RACE for BP133312 |
| NtBP1333-333F-5RACE |
GAGAATCTCTCATCAGCCAGATGTTC |
3’-RACE for BP133312 |
| NtBP1333-375F-nested |
CAAACCTGTTGGTGGGGTACGAAT |
3’-RACE for BP133312 |
| NtBP1333-F1 |
AGACGCGCATAATTCGCATC |
Sequencing |
| NtBP1333-R1 |
TGAGCTCTCGTTTGGTAATAAG |
Sequencing |
| LeBI9315-507R-5RACE |
CATCTGCCTCTGTCAACTTTGTTGCA |
5’-RACE for LeBI931567 |
| LeBI9315-165R-nested |
CCTCCTGAGCAATATTCTCCAGAGA |
5’-RACE for LeBI931567 |
| LeBI9315-482F-3RACE |
TGCAACAAAGTTGACAGAGGCAGATG |
3’-RACE for LeBI931567 |
| LeBI9315-564F-nested |
ATGTCGGGAGTATGTGCTGCATCTG |
3’-RACE for LeBI931567 |
| LeBI9315-F1 |
CTGAGAAAGTTCTTGCAAACG |
Sequencing |
| LeBI9315-F2 |
GAACAGATGAATCAGAAGATG |
Sequencing |
| LeBI9315-F3 |
AAAGCACTCCATATAAACACAG |
Sequencing |
| LeBI9315-R1 |
GGTATACATCAAGTCCACCAT |
Sequencing |
| LeBI9315-R2 |
GAGGGAGTATGCTCTCATG |
Sequencing |
| Kp-NbMAPKKKa-1F |
GGGGTACCGAATGCCTGCTTGGTGGGGAA |
Full-length ORF of NbMAPKKKα |
| Xh-NbMAPKKKa-1836R |
GGCTCGAGTGCTAAAGAATTGGTCTTAGTTTTG |
Full-length ORF of NbMAPKKKα |
| Kp-NbMAPKKKb-1F |
CCGGTACCGAATGCATCGATTGCCAGGAATTTTTGC |
Full-length ORF of NbMAPKKKβ |
| EcV-NbMAPKKKb-1695R |
GGGATATCTTTAAAGCCTCTTGCCCAGATTTTG |
Full-length ORF of NbMAPKKKβ |
| Kp-NbMAPKKKg-1F |
GGGGTACCGAATGCGTTGGTGGCAGAACG |
Full-length ORF of NbMAPKKKγ |
| Xh-NbMAPKKKg-1956R |
GGCTCGAGTGCTACCTCTCTAGAGATAAACG |
Full-length ORF of NbMAPKKKγ |
| Kp-NbMAPKKKbkinase-F |
CCGGTACCGAATGTACTGGGACAAAGGTGATCT |
Kinase domain of NbMAPKKKβ |
| EcV-NbMAPKKKbkinase-R |
GGGATATCTTTACACAAAAGGATGCTCCAAGA |
Kinase domain of NbMAPKKKβ |
| Kp-NbMAPKKKgkinase-F |
GGGGTACCGAATGTGGCAAAAAGGGAAGCTTATTG |
Kinase domain of NbMAPKKKγ |
| Xh-NbMAPKKKgkinase-R |
GGCTCGAGTGTTACATAAATCGATGTTCCAATAAC |
Kinase domain of NbMAPKKKγ |
| Kp-NbMAPKKKekinase-F |
GGGGTACCGAATGAAATATATGCTCGGAGATGAG |
Kinase domain of NbMAPKKKε2 |
| Xh-NbMAPKKKekinase-R |
GGCTCGAGTGTTATATCCATGGATGTGAAAGTAG |
Kinase domain of NbMAPKKKε2 |
| NbMAPKKKb-K381M-F |
TTTTGCTGTCATGGAAGTGTCATTACTTGATCA |
K → M mutant of NbMAPKKKβ |
| NbMAPKKKb-K381M-R |
ATGACACTTCCATGACAGCAAAAAAGAAACCG |
K → M mutant of NbMAPKKKβ |
| NbMAPKKKg-K374M-F |
CTGGAGCTTTATGTGCGATGATGGAAGTTGAATT ATTACCGGA |
K → M mutant of NbMAPKKKγ |
| NbMAPKKKg-K374M-R |
TCCGGTAATAATTCAACTTCCATCATCGCACATAA AGCTCCAG |
K → M mutant of NbMAPKKKγ |
| NbMAPKKKa-255F |
GGTTGTTTTGGGATGTGGGGTCAG |
Real-time RT-PCR for NbMAPKKKα |
| NbMAPKKKa-393R |
CAGTGGGCTCAACCTATTATCGCC |
Real-time RT-PCR for NbMAPKKKα |
| NbMAPKKKb-1179F |
CACAAGGCAGATTTTACATGGTTTG |
Real-time RT-PCR for NbMAPKKKβ |
| NbMAPKKKb-1286R |
AGCTTGACCGATCCGTTAGCA |
Real-time RT-PCR for NbMAPKKKβ |
| NbMAPKKKg-903F |
CCGTGAGTGTAGTGCTCAGGGTAA |
Real-time RT-PCR for NbMAPKKKγ |
| NbMAPKKKg-984R |
TGCCGTAGGCTGCTGTGATG |
Real-time RT-PCR for NbMAPKKKγ |
| Nb18S-193F |
ATACGTGCAACAAACCCCGAC |
Real-time RT-PCR for Nb18S rRNA |
| Nb18S-280R | TGAATCATCGCAGCAACGG | Real-time RT-PCR for Nb18S rRNA |