Table 1. Segregation of male fertile and sterile plants of Salvia miltiorrhiza (Sh-B) in backcross and testcross populations tested over three years.
Cross* | Year | No. of plants observed | Segregation ratio | Probability | |
Fertile plants | Sterile plants | ||||
(Sh-B×A-20) F1 | 2007 | 155 | 148 | 1:1 | 0.17 |
(Sh-B×A-249)F1 | 2007 | 174 | 169 | 1:1 | 0.22 |
(Sh-B×A-107)F1 | 2007 | 207 | 193 | 1:1 | 0.13 |
(Sh-B×A-20) BC1F1 | 2008 | 206 | 195 | 1:1 | 0.21 |
(Sh-B×A-249)BC1F1 | 2008 | 193 | 189 | 1:1 | 0.29 |
(Sh-B×A-107)BC1F1 | 2008 | 205 | 198 | 1:1 | 0.18 |
(Sh-B×A-20) BC2F2 | 2009 | 206 | 200 | 1:1 | 0.15 |
(Sh-B×A-249)BC2F2 | 2009 | 194 | 185 | 1:1 | 0.22 |
(Sh-B×A-107)BC2F2 | 2009 | 195 | 193 | 1:1 | 0.62 |
Sh-B×A-20Sh-BA | 2009 | 215 | 211 | 1:1 | 0.22 |
Sh-B×A-107Sh-BA | 2009 | 209 | 202 | 1:1 | 0.18 |
Sh-B×A-249Sh-BA | 2009 | 207 | 199 | 1:1 | 0.15 |
A-20 | 2008 | 120 | 0 | ||
A-249 | 2008 | 140 | 0 | ||
A-107 | 2008 | 150 | 0 | ||
A-20Sh-BA | 2008 | 180 | 0 | ||
A-107Sh-BA | 2008 | 170 | 0 | ||
A-249Sh-BA | 2008 | 190 | 0 |
A-20, A-249 and A-107 were sterile and inbred lines of Salvia miltiorrhiza with different biological characteristics; BC1F1 represented F1 progeny with one backcross; BC2F2 represented F2 progeny with twice backcross; represented self pollination; A-20Sh-BA represented half of fertile plants obtained from sterile seeds after hybridization between A-20 × Sh-B; A-20Sh-BA was self pollination of A-20Sh-BA progeny.