Table 2. Fitting parameters derived from the analysis of initial nucleation rates at 37°C.
[NaCl] | KU a | KA (×10−3) | KI | kF (min−1×10−3) |
N47A Spc-SH3 | ||||
50 mM | 0.071 (fixed) | 246 ± 50 | 0.0024 ± 0.0010 | 2.0 ± 3.6 |
100 mM | 0.117 (fixed) | 0.0058 ± 0.0010 | 17.6 ± 2.8 | |
200 mM | 0.165 (fixed) | 0.0109 ± 0.0018 | 38.7 ± 7.3 | |
N47A-K43A Spc-SH3 | ||||
100 mM | 0.495 (fixed) | 246 (fixed) | 0.036 ± 0.010 | 34.6 ± 7.8 |
Calculated from the unfolding curves measured by DSC. Data of N47A Spc-SH3 were taken from reference [23].