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. 2012 Nov 27;7(11):e49745. doi: 10.1371/journal.pone.0049745

Figure 4. Type III (T3SS) and VI (T6SS) secretion systems are not required for virulence in Drosophila.

Figure 4

(A) Survival of wild-type or Dif; Rel mutant flies infected with exponential-phase wild-type, T3SS-mutant (AH174) or T6SS-mutant B. thailandensis, maintained at 25°C, and counted at least every hour. (B) Median survival times from [A]. Statistical significance was determined using Mann-Whitney test; ** p<0.002 and *** p<0.0005. (C) Survival of wild-type and Dif; Rel mutant flies after infection with wild-type B. thailandensis E264 at 18°C. Under these conditions, Dif; Rel mutants were significantly shorter-lived. Statistical significance between the survival curves of infected WT and mutant flies was determined using Log-rank analysis (Mantel-Cox); p<0.0001. The data showing the WT Drosophila subset is the same as in [Fig. 2A]; all results shown here were obtained at the same time. (D) Proliferation of the T3SS mutant (AH174), and complemented AH186 mutant in WT Drosophila. Statistical significance of bacterial growth between time points was determined using Mann-Whitney test.