Table 5.
Sequence Based Polymorphic (SBP) markers generated for thePSS1region
| Name | 1Primer | 2Enzyme | AmpliconSize (bp) |
|---|---|---|---|
| SBP_22.95 |
F: GGAGGTTCCGTTACTC TTACTG R: CCACCGGAA GACGACGACTCTTC |
RsaI |
309 |
| SBP_22.98 |
F: CGACGTCACACTCTCC GTTA R: CCGATGATGGA GAAGGAAAA |
TaqI |
230 |
| SBP_23.06 |
F: AAATTGGGGACACCA ACAAA R: GGTCCTCCTG GGAGAAAGAT |
Tsp509I |
180 |
| SBP_23.09 |
F: TCGAATGATCCTTTCC TTTCA R: GCTTTTGCGA AAATGGGATA |
TaqI |
235 |
| SBP_23.46 | F: GACCAAATGTCTCTGA GATGTTC R: ACCCAAGG CGGTGTTGGCGAAAG | TaqI | 520 |
1Primers: F, forward primer; R, reverse primer. 2Restriction endonucleases used for individual CAPS markers are shown