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Immunodetection of5mC in (a) wild type S2 and (b) E2 pachytene chromosome spreads from meiocytes. 4’,6-Diamidino-2-Phenylindole (DAPI) staining indicated in blue, and anti-5mC detected using Alexa-488 conjugated secondary antibody indicated in green. E2 (b) shows reduced 5mC signal in non-heterochromatic regions compared with S2 (a).
