Table 1. Proteins Expressed Differently in Mitochondrial Fraction Isolated from the Brain of WT and p53^(−/−) mice.

Spot	Protein Identified	Accession #	Coverage	Number of identified peptidesa	Score	MW (kDa)	pI	P valueb	Foldc
1	Guanine nucleotide-binding protein G (o) subunit alpha	P18872	12.15	3	24.11	40.1	5.53	0.0019	212 ↑ p53KO
2	ATP synthase subunit beta, mitochondrial	P56480	4.54	2	18.16	56.3	5.34	0.0035	125 ↑ p53KO
3	Heat shock cognate 71 kDa protein	P63017	37.31	20	196.60	70.8	5.52	0.002	212 ↑ p53KO
4	Aldehyde dehydrogenase family 5, subfamily A1	B2RS41	14.72	6	36.70	55.9	8.25	0.0009	131 ↑ p53KO
5	Glutamate dehydrogenase 1, mitochondrial	P26443	26.34	13	78.69	61.3	8.00	0.0076	131 ↑ p53KO
6	Isoform mithocondrial of Fumarate hydratase	P97807-2	25.57	8	62.73	50.0	7.94	0.0019	325 ↑ p53KO
7	Acetyl-CoA acetyltransferase	Q8QZT1	26.89	8	50.64	44.8	8.51	0.00079	166 ↑ 53KO
8	Isoform Mt-VDAC1 of Voltage- dependent anion-selective channel protein 1	Q60932-2	38.16	7	74.55	30.7	8.54	0.0027	201 ↑ p53KO
9	Aspartate aminotransferase	P05202	43.72	17	174.33	47.4	9.00	0.0037	210 ↑ p53KO
10	Mn Superoxide dismutase	P09671	13.96	4	43.39	24.6	8.62	0.0026	133 ↑ 53KO
11	Cytochrome b-c1 complex Rieske subunit	Q9CR68	26.28	7	70.31	29.3	8.70	0.0030	252 ↑ 53KO
12	Thioredoxin-dependent peroxide reductase	P20108	28.40	7	41.17	28.1	7.58	0.0015	253 ↑ 53KO

^aThe number of peptide sequences identified by nanospray ESI-MS/MS of tryptic peptides.

^bThe fold-change in spot density from p53^(−/−) mice compared to wt. The arrow indicates the direction of change.

^cThe p-value associated with fold-change calculated using a Student's t-test.