Figure 3.

FISH detection of viral RNA in salivary glands of Aedes albopictus by confocal microscopy. FISH was carried out on salivary glands dissected from Ae. albopictus females at 14 days post-infection with blood-meals containing DENV-2 Bangkok strain, using the three DENV-Probes simultaneously. Viral RNA is detected (green) in the distal zone of the lobe close to the salivary duct. No signal was detected in control females engorged with an uninfected blood-meal. Bar, 500 μm. A global view of salivary glands is provided in the supplementary Figure S2.