Table 3.
Cytokine levels in serum and in cell supernatants before and after doxycycline and placebo treatment
| Cytokine | Antigen | Doxycycline median pg/ml (Interquartile range) | Placebo median pg/ml (Interquartile range) | Detectable (%) | ||
|---|---|---|---|---|---|---|
| |
|
Before |
After |
Before |
After |
|
|
Serum |
|
|
|
|
|
|
| IL-1β |
|
0.43 (0.13-1.50) |
0.60 (0.13-1.10) |
0.52 (0.13-0.70) |
0.60 (0.13-0.84) |
68 |
| IL-6 |
|
0.19 (0.19-1.60) |
0.19 (0.19-2.70) |
0.19 (0.19-2.70) |
0.19 (0.19-3.20) |
43 |
| IL-8 |
|
8.15 (6.0-12.9) |
9.78 (7.70-14.3) |
10.6 (7.40-14.9) |
10.1 (7.90-13.7) |
100 |
| GM-CSF |
|
0.75 (0.75-9.85) |
0.75 (0.75-9.10) |
0.75 (0.75-10.2) |
0.75 (0.75-11.5) |
40 |
| IL-10 |
|
0.15 (0.15-1.80) |
0.15 (0.15-1.72) |
0.15 (0.15-1.30) |
0.15 (0.15-1.97) |
45 |
|
Supernatants |
|
|
|
|
|
|
| IL-1β |
OF |
267 (147–416) |
276 (178–353) |
258 (160–372) |
257 (212–325) |
100 |
| |
LPS |
373 (297–578) |
336 (273–459) |
384 (306–464) |
376 (272–417) |
100 |
| IL-6 |
OF |
8495 (5577–11552) |
10018 (7283–11436) |
9017 (7893–12936) |
10549 (6765–11870) |
97 |
| |
LPS |
7457 (5522–9344) |
7043 (6173–9401) |
7339 (5640–9010) |
7129 (6164–8376) |
100 |
| IL-8 |
OF |
234726 (182996–353519) |
256238 (224077–323425) |
264450 (187637–377221) |
254926 (343734) |
100 |
| |
LPS |
170005 (84896–191690) |
158091 (116470–210940) |
159121 (119796–175857) |
153546 (203419) |
100 |
| |
unstim. |
949 (425–3337) |
887 (412–2657) |
966 (580–2637) |
728 (443–1324) |
100 |
| TNF |
OF |
49.4 (41.8-72.4) |
51.8 (26.9-210) |
91.2 (66.7-141) |
69.3 (25.8-152) |
77 |
| |
LPS |
45.0 (23.8-61.3) |
45.4 (31.7-56.4) |
36.7 (27.1-57.5) |
37.1 (26.4-51.9) |
77 |
| IL-10 |
OF |
50.6 (41.8-84.7) |
59.3 (48.4-84.7) |
64.4 (46.3-100) |
67.3 (47.0-86.3) |
82 |
| LPS | 177 (130–272) | 184 (114–259) | 201 (154–255) | 181 (119–280) | 98 | |
The table shows median values for spontaneous cytokine levels in serum and antigen-stimulated (and unstimulated IL-8 levels) cytokine levels in peripheral blood mononuclear cell supernatants, before and after doxycycline and placebo treatment from all 15 included patients. IL, interleukin; GM-CSF, granulocyte macrophage colony-stimulating factor; TNF, tumor necrosis factor; OF, outer surface–enriched fraction of B. garinii strain Ip90; LPS, lipopolysaccharide; unstim., unstimulated.