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. 2012 Nov 27;7(11):e49962. doi: 10.1371/journal.pone.0049962

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© 2012 Tatura et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Relative quantification of Treg cells by FOXP3 Treg-specific demethylated region (TSDR) real-time quantitative polymerase chain reaction (quantitative analysis of methylated alleles, QAMA) B Relative quantification of Treg cells (characterized as CD4⁺ CD25^hiCD127^low) by flow cytometry C Representative quantification of one septic patient and healthy subject by flow cytometry. Setting of the CD25 and CD127 flow cytometer gates are shown.