Figure 6. Runx3 depleted embryos can be rescued by runx3 and egr1 mRNA.
(A–F) Head cartilages were stained with Alcian Blue in morpholino treated larvae at 4 dpf; ventral views are shown. (A) MOcon 2 ng injected larvae. (B) MOrunx3 2 ng injected larvae do not develop viscerocranium. (C) 100 pg of runx3 mRNA do not affect 4 dpf old larvae cartilage morphology. (D) Injection of 100 pg runx3 mRNA rescues 89% of MOrunx3 2 ng injected eggs. (E) 80 pg of egr1 mRNA. (F) Injection of 80 pg egr1 mRNA rescues 62% of MOrunx3 2 ng injected eggs.