Table 2. Chimerism and germ line transmission of DBA/2J ES cells.

Cell line, culture condition	% of chimeric mice born (n)	% male chimeras	% germline chimeras	transmission rate (male only)
AC203, 1i	16% (19)	67%	100%	100%
AC216, 1i	38% (13)	100%	20%	100%
AC173, 3i	59% (37)	68%	92%	97%
AC175, 3i	46% (13)	100%	67%	100%

Four DBA/2J ES cell lines (two derived from each media type, 1i and 3i) were assessed for capacity to contribute to the germ line. These data are based on standard microinjection of ES cells into C57BL/6J host blastocysts. Additional data, including laser assisted microinjection data can be found in Table S1. Details are provided for: the number of chimeric mice (determined based on coat showing at least partial dilute brown agouti color, column 2); the % of those chimeric mice that were male (column 3); the % of male chimeric mice that sired offspring with 100% dilute brown agouti color (column 4); and the % of offspring that were completely dilute brown agouti (transmission rate, column 5).