Figure 7. ATXN2L reduction affects P-body formation.

HeLa cells were left untreated (mock) or transfected with siRNAs against ATXN2L or ATXN2 transcripts, or non-targeting control siRNAs. The cells were fixed 72 hours post transfection, and analyzed A) by confocal microscopy or B–D) by quantitative high-content screening microscopy. In A) cells were stained with an antibody detecting DCP1. In B–D) cells were stained with antibodies detecting DCP1 and DDX6. B) Cell numbers and size of nuclei of analyzed cells. C, D) Quantification of DCP1- and DDX6-positive P-bodies regarding number and size. Results are expressed as mean ± SD from one representative experiment, n = 5 replicate wells, *p<0.05; **p<0.01; ***p<0.001, One-way ANOVA with Tukey’s Multiple Comparison post test.