Table I.
Experiment | Isoprene
Synthase Activity
|
|||||
---|---|---|---|---|---|---|
Thylakoid bound | Percent | Soluble | Percent | Total | Percent | |
pmol isoprene min−1 | pmol isoprene min−1 | pmol isoprene min−1 | ||||
1 | 1146 | 45 | 1418 | 55 | 2564 | 100 |
2 | 740 | 47 | 821 | 53 | 1561 | 100 |
3 | 1368 | 44 | 1742 | 56 | 3110 | 100 |
4 | 840 | 25 | 2583 | 75 | 3423 | 100 |
Average | 1024 | 40 | 1641 | 60 | 2665 | 100 |
sd | 287 | 11 | 735 | 11 | 817 |
Mature willow leaves (10 g) were fractionated into total thylakoid-bound and soluble isoprene synthase activities, as described in Methods. Each value is the average of duplicate samples.