Fig. 2.

Focused gene expression profiling of PMA differentiated THP-1 cells treated with rHSP27. NF-κB pathway-focused qPCR arrays were used to screen the expression of genes involved in this pathway. THP-1 macrophages were treated with rHSP27 (9.6 μM) supplemented with polymyxin B (PMB, 10 μg/mL). The delta delta Ct method was then used to calculate the fold change of genes that are up/down-regulated in rHSP27-treated macrophages compared to control. Genes with fold change >5 and a p value < 0.05 were considered to be significant and are shown on a logarithmic scale